Molecular Cloning and Expression of a Squalene Epoxidase Gene from Ilex cornuta
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چکیده
منابع مشابه
Molecular Characterization of a Squalene epoxidase Gene in Dermatophyte Pathogen Trichophyton tonsurans
Background: Trichophyton tonsurans is one of the dermatophyte fungi which invades the skin and hair of human. Several properties of this fungus have been investigated so far. However a few studies were carried out in the field of molecular biology of this fungus. In the present study, we tried to identify the Squalene epoxidase gene which is related to synthesis of ergosterol in this fungus. Me...
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Background: Stresses (such as drought, salt, viruses, and others) seriously affect plant productivity. To cope with these threats, plants express a large number of genes, including several members of ERD (early responsive to dehydration) genes to synthesize and assemble adaptive molecules. But, the function of ERD3 gene hasn’t been known so far.Objectives:</strong...
متن کاملPurification and characterization of recombinant squalene epoxidase.
Recombinant rat squalene epoxidase (rSE) was expressed in E. coli and purified to an apparent homogeneity. This expression system was constructed using squalene epoxidase (SE) cDNA in which nucleotides coding 99 amino acids in the N-terminal were deleted and nucleotides coding hexa-histidine in the C-terminal were added. Purification was carried out using Ni-chelate affinity agarose and Cibacro...
متن کاملSqualene Epoxidase of Rat Liver*
The microsomal enzyme system from rat liver which catalyzes the epoxidation of squalene to 2,3-oxidosqualene (squalene epoxidase) has been investigated further with 10 ,ll-dihydrosqualene as the artacial substrate. Liver supernatant contributes two essential components to the epoxidase system, one heat stable and the other heat labile (YAMAMOTO, S., AND BLOCH, K. (1970) J. Biol. Chem. 245, 1670...
متن کاملmolecular characterization of a squalene epoxidase gene in dermatophyte pathogen trichophyton tonsurans
background: trichophyton tonsurans is one of the dermatophyte fungi which invades the skin and hair of human. several properties of this fungus have been investigated so far. however a few studies were carried out in the field of molecular biology of this fungus. in the present study, we tried to identify the squalene epoxidase gene which is related to synthesis of ergosterol in this fungus. me...
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ژورنال
عنوان ژورنال: Biotechnology(Faisalabad)
سال: 2017
ISSN: 1682-296X
DOI: 10.3923/biotech.2018.26.34